Extracellular matrices (ECM) used for cell culture is a temperature-sensitive protein mixture that supports cell attachment, differentiation, and both 2D and 3D culture systems, including organoid models. Because many basement membrane matrix products (such as EHS-derived matrices like Geltrex™) begin to polymerize above approximately 10–15°C and rapidly increase in viscosity as they warm, small handling
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Source: Thermo Fisher Blog